![]() ![]() ![]() Our results showed the first visualization of complete cholinergic neurons, including axons projecting to the OB, and also revealed frequent axonal branching within the OB where it innervated multiple glomeruli in different areas. ![]() To further clarify the function of cholinergic neurons, we performed confocal laser scanning microscopy to investigate whether other neurotransmitters are present within cholinergic axons in the OB. We also examined the ultramicrostructure of synapses by electron microscopy tomography. We labeled cholinergic neurons by infection with virus vector and then reconstructed them three-dimensionally. Single-cell imaging of a specific neuron within dense fibers is critical to evaluate the structure and function of the neural circuits. In this study, we focused on cholinergic regulation of the OB and analyzed neural morphology with a particular emphasis on the projection pathways of cholinergic neurons. ![]() Cholinergic neurons projecting from the nucleus of the horizontal limb of the diagonal band of Broca and the magnocellular preoptic nucleus are one of the primary centrifugal inputs to the OB. Odor information is regulated by olfactory inputs, bulbar interneurons, and centrifugal inputs in the olfactory bulb (OB). ![]()
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